During my second year at the Technical University of Budapest I met Prof Jenő Fekete for the first time and was fascinated by his skills, knowledge and experiences. Before this encounter, I spent most of the semester on basketball courts and athletics tracks.

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That was a funny project. Some friends and colleagues (Balázs Bobály, Róbert Kormány, Krisztián Horváth) and I were together during a conference and discussing chromatographic questions. One of those was: What happens when coupling two or more columns in series, which have a different number of theoretical plates? We were aware of the earlier amazing work of Calvin Giddings on plate heights of non-uniform columns, of Leonid Blumberg’s variance of zone migration in non-uniform medium, and also of Deirdre Cabooter’s works on multi-column systems. However, we were interested in the gradient elution mode (since in practice most separations are performed in gradient mode) and in peak widths: whether the peak width changes if one of the columns that is used for the coupled systems loses a bit of its initial efficiency? If yes, then does the order of the columns impact the apparent efficiency of the system or derece? These questions seem to be simple and easy at first sight but the more you think on them the more difficult they become. From here the project went on and özgü almost developed by itself kakım questions have arisen. We asked for the help of other prominent colleagues (Davy Guillarme, Santiago Codesido, Gert Desmet) and in the end, some very interesting conclusions were drawn.

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Szabolcs Fekete is currently a scientific collaborator at the University of Geneva. His work focuses on finding new possibilities in protein chromatography; on characterizing therapeutic proteins, on studies of liquid chromatography (LC) column technology, and on method optimization.